TY  - BOOK
AU  - Chua,R.W.
AU  - Song,K.P.
AU  - Ting,A.S.Y.
TI  - Characterization and antimicrobial activities of bioactive compounds from endophytic Trichoderma asperellum isolated from Dendrobium orchids
T2  - Biologia, 79(2), p.569-584, 2024
KW  - ANTIMICROBIAL ACTIVITIES
KW  - BIOACTIVE COMPOUNDS
KW  -  DENDROBIUM ORCHIDS
KW  -  ENDOPHYTIC FUNGI
KW  - TRICHODERMA ASPERELLUM
N1  - Artículo
N2  - Endophytic Trichoderma asperellum (D7) from Dendrobium orchids was explored for its antimicrobial potential through characterization and identification of bioactive compounds. Bio-guided fractionation of crude extracts of T. asperellum (D7) afforded 7 fractions with antimicrobial activities against bacterial pathogens (Bacillus cereus and Pseudomonas aeruginosa) and a plant pathogen (Ganoderma boninense). Among the fractionated extracts, fraction 1 was most effective towards P. aeruginosa (MIC: 1.25 mg/mL) while fraction 4 had the strongest inhibitory activities against B. cereus (MIC: 2.5 mg/mL) and G. boninense (50.62 percent inhibition). Liquid chromatography-mass spectrometry (LCMS) analysis led to the identification of antimicrobial compounds from fraction 1 (paeoniflorin, kanzonol N, 2-nonynoic acid, fenpropidin, (+)-tetrandrine, 12(13)-EpOME-d, rifamycin, linoleic acid(d4) and 3α,12α-dihydroxy-5β-chol-8(14)-en-24-oic acid) and fraction 4 (morpholine, epothilone A, tetroquinone, loperamide, 2,5-dimethoxycinnamic acid, 3,4-dihydroxyphenyl ethanol, pyropheophorbide A, streptomycin, 5-hydroxystreptomycin, rifamycin, leucomycin A1, sophoradiol, 5R-hydroxy-hexanoic acid, 2-undecanol, 3-methyl-2-quinoxalinone, scandenin and lumichrome) of T. asperellum (D7). Several of the antimicrobial compounds were revealed for the first time for endophytic T. asperellum (D7). The identification of bioactive compounds from T. asperellum (D7) would contribute to the development of natural antimicrobials, agrochemicals, fungicides and pesticides
UR  - https://drive.google.com/file/d/1IUxU3OKl4ue2IVRGEQPMc5ZG4Q2UHxQ9/view?usp=drive_link
ER  -