Chemical Variation and Biological Properties of the Essential Oil and Main Volatiles of Pimenta dioica Harvested in the Northern Highlands of Puebla, Mexico 

 - Chemistry & Biodiversity, https://doi.org/10.1002/cbdv.202402843 .
<br/><br/> Artículo 
<br/><br/> The chemical variation of the annual essential oils from Pimenta dioca (PdEOs) grown in the Northern Highlands of Puebla, Mexico, and their biological activities were determined. Eugenol (>50 percent) and beta-myrcene (>16 percent) were the main volatiles contained in the annual PdEOs (2022-2024) extracted from fruit and leaves. PdEOs showed inhibitory properties on human pancreatic lipase (IC50, 33-44 µg/mL) through mixed type inhibition with clear eugenol involvement (IC50, 47 µg/mL). The oral administration of PdEOs and eugenol combined with corn oil on ICR mice (p < 0.01), confirmed a strong anti-hypertriglyceridemic activity at 3 and 4.5 h post-treatment. PdEOs showed low toxicity in 3T3 fibroblasts and probiotics (IC50 >500 µg/mL), whereas high lethal dose in ICR mice (LD50 >8000 mg/kg BW). Eugenol, beta-myrcene and PdEOs decreased Helicobacter pylori viability acting as mixed inhibitors on urease. Results of anti-lipase and anti-H.pylori urease assays were endorsed by docking calculations. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>ANTI-HELICOBACTER PYLORI<br/>ANTI-LIPASE<br/>BETA-MYRCENE<br/>ESSENTIAL OIL<br/>EUGENOL<br/>PIMENTA DIOICA