TY  - BOOK
AU  - Chi,C.M.;Vits,H.;Staba,E.J.;Cooke,T.J.;Hu,W.S.
TI  - Morphological kinetics and distribution in somatic embryo cultures
T2  - Biotechnology and Bioengineering, 44, p.368-378, 1994
KW  - DAUCUS CAROTA L
KW  - EMBRYOS
KW  - KINETICS
KW  - MORPHOLOGY
KW  - PATTERN RECOGNITION
KW  - IMAGE ANALYSIS
N1  - Artículo
N2  - The environmental effects on developing somatic embryos should be characterized not only by the growth based on biomass, but also by the morphological properties and size. We have previously developed a discrete classifier to separate developing embryos into distinct morphological classes. In this study, a continuous descriptor using the distributions of magnitude of features representing morphological characteristics and size information was used to describe the developing embryo populations. The identity of the population was examined by comparing either the distributions of all features or of key features. The method was applied to characterize the kinetics of carrot embryo populations cultivated in the presence and absence of triiodobenzoic acid (TIBA), an inhibitor of auxin polar transport. Optimal sample size for morphological characterization was determined by the invariance of feature distributions with further increase in sample size. The overall growth and substrate consumption kinetics were only slightly affected by the presence of TIBA. However, the distribution of morphological features was significantly affected. The features showing the highest statistical significance were related to those corresponding to the roughness. The continuous descriptor for characterizing developing embryo population is potentially useful for quality control in large-scale operations
UR  - https://drive.google.com/file/d/1tDb2iiAZCV_aJazRUUfGPpiBp219Cu-a/view?usp=drive_link
ER  -