TY  - BOOK
AU  - Bootman,Martin D.
AU  - Bultynck,Geert
AU  - Parys,Jan B.
AU  - Yule,David I.
TI  - Calcium techniques: a laboratory manual 
SN  - 9781621820789 (cloth)
U1  - C 572.516 
PY  - 2014///
CY  - New York
PB  - Cold Spring Harbor Laboratory Press, 
KW  - CALCIO, CANALES
KW  - MANUALES DE LABORATORIO
KW  - CALCIO, SEÑALES
KW  - CALCIO
KW  - FISIOLOGIA
KW  - FLUOROMETRIA
KW  - METODOS
N1  - Incluye referencias bibliográficas e índice
N2  - Life begins with a surge of calcium ions (Ca2+) at fertilization, and thereafter, Ca2+ signaling influences nearly every aspect of mammalian development and physiology, from gene expression and cell proliferation to ra úsele contraction and nerve impulses. To créate spatiotemporally distinct Ca2+ signáis, cells use a variety of mechanisms to recognize, transport, and buffer Ca2+. Thus, a diverse range of reliable experimental techniques is necessary to study the movement of Ca2+ and the various effectors involved. This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principies and applica-tions of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imag-ing modalities to characterize Ca2+ signáis. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and meth-ods to investígate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and inter-cellular signáis are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice. Specific chapters are devoted to Ca2+ signaling techniques in nonmammalian systems, such as plants, yeast, zebrafish, and Xenopus. Methods for assessing Ca2+-binding kinetics and strategies for developing mathematical models of Ca2+ signaling are also included. Thus, this manual is a comprehensive laboratory resource for biochemists, cell and developmental biologists, and physiologists who are using or looking to expand their repertoire of Ca2+ techniques
UR  - https://www.cicy.mx/sitios/sib/doctoelectronico/8275.pdf
ER  -