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DNA Methylation and Transcriptomic Next-Generation Technologies in Cereal Genomics.

Tipo de material: TextoTextoSeries ; In Cereal Genomics. Humana, New York, NY., p.65-84, 2020Trabajos contenidos:
  • Soto Cardinault, C. G
  • Duarte Aké, F
  • De La Peña, C
  • Góngora Castillo, E
Tema(s): Recursos en línea: Resumen: RNA sequencing (RNA-seq)coupled to DNA methylation strategies enables the detection and characterization of genes which expression levels might be mediated by DNA methylation. Here we describe a bioinformatics protocol to analyze gene expression levels using RNA-seq data that allow us to identify candidate genes to be tested by bisulfite assays. The candidate methylated genes are usually those that are low expressed in a particular condition or developmental stage.
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RNA sequencing (RNA-seq)coupled to DNA methylation strategies enables the detection and characterization of genes which expression levels might be mediated by DNA methylation. Here we describe a bioinformatics protocol to analyze gene expression levels using RNA-seq data that allow us to identify candidate genes to be tested by bisulfite assays. The candidate methylated genes are usually those that are low expressed in a particular condition or developmental stage.

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