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Metabolic engineering of the indole pathway in Catharanthus roseus hairy roots and increased accumulation of tryptamine and serpentine

Tipo de material: TextoTextoSeries ; Metabolic Engineering, 6(4), p.268-276, 2004Trabajos contenidos:
  • Hughes, E.H
  • Hong, S-B
  • Gibson, S.I
  • Shanks, J.V
  • San, K-Y
Recursos en línea: Resumen: Transgenic hairy roots of Catharanthus roseus were established with glucocorticoid inducible tryptophan decarboxylase (TDC)expression alone or in combination with inducible expression of a feedback-resistant anthranilate synthase alpha subunit (AS)from Arabidopsis. Northern blot analysis confirmed transcription of the anthranilate synthase gene upon induction in the double line (TDC+AS)and in vitro enzyme assays confirmed increased resistance to feedback inhibition by tryptophan. In TDC enzyme assays, increases of 48
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Transgenic hairy roots of Catharanthus roseus were established with glucocorticoid inducible tryptophan decarboxylase (TDC)expression alone or in combination with inducible expression of a feedback-resistant anthranilate synthase alpha subunit (AS)from Arabidopsis. Northern blot analysis confirmed transcription of the anthranilate synthase gene upon induction in the double line (TDC+AS)and in vitro enzyme assays confirmed increased resistance to feedback inhibition by tryptophan. In TDC enzyme assays, increases of 48

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