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In vitro response of leaf tissues from Lolium multifloruma comparison with leaf segment position, leaf age and in vivo mitotic activity

Tipo de material: TextoTextoSeries ; Theoretical and Applied Genetics, 73, p.286-291, 1986Trabajos contenidos:
  • Joarder, O.I
  • Joarder, N.H
  • Dale, P.J
Tema(s): Recursos en línea: Resumen: Immature gramineous leaves provide a convenient system for comparing the response of cells in culture with their state of differentiation. Callusing frequency is compared with leaf segment position, leaf age and in vivo mitotic activity in Lolium multiflorum. (1)In a succession of one millimeter sections from the immature leaf base, callus was formed from the first and second sections but not the third or subsequent sections. The frequency of those explants callusing decreased with distance from the base of the leaf and with leaf age (or leaf extension growth). (2)In vivo, the proportion of cells in mitosis declined from around 10-14
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Immature gramineous leaves provide a convenient system for comparing the response of cells in culture with their state of differentiation. Callusing frequency is compared with leaf segment position, leaf age and in vivo mitotic activity in Lolium multiflorum. (1)In a succession of one millimeter sections from the immature leaf base, callus was formed from the first and second sections but not the third or subsequent sections. The frequency of those explants callusing decreased with distance from the base of the leaf and with leaf age (or leaf extension growth). (2)In vivo, the proportion of cells in mitosis declined from around 10-14

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