Image from Google Jackets

Differential Display Identifies Developmentally Regulated Genes during Somatic Embryogenesis in Eggplant (Solanum melongena L.)

Tipo de material: TextoTextoSeries ; BioChemical and Biophysical Research Communications , 213(2), p.376-382, 1995Trabajos contenidos:
  • Takahiro, M
  • Afele, J.C
  • Saito, T
  • Kayano, T
  • Tabel, Y
  • Takaiwa, F
  • Takayanagi, K
  • Takayanagi, K
Recursos en línea: Resumen: Changes in gene expression patterns during early somatic embryogenesis of eggplant (Solanum melongena L.)were characterized by means of differential display. Eight products of polymerase chain reaction (PCR)derived from newly expressed mRNAs after four days of culture were identified and cloned. One clone, pTM002, was found to contain a 416 bp insert and identical to the proximal arbitrary primer at both ends. Based on the terminal sequences, reverse-transcription PCR (RT-PCR)was carried out to monitor the expression level of pTM002. The results indicated that the message of pTM002 was increased to correlate with the intensities obtained in the differential display. The significance of this method in investigation of differentially expressed genes is also discussed.
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Collection Call number Status Date due Barcode
Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-6484 (Browse shelf(Opens below)) Available

Changes in gene expression patterns during early somatic embryogenesis of eggplant (Solanum melongena L.)were characterized by means of differential display. Eight products of polymerase chain reaction (PCR)derived from newly expressed mRNAs after four days of culture were identified and cloned. One clone, pTM002, was found to contain a 416 bp insert and identical to the proximal arbitrary primer at both ends. Based on the terminal sequences, reverse-transcription PCR (RT-PCR)was carried out to monitor the expression level of pTM002. The results indicated that the message of pTM002 was increased to correlate with the intensities obtained in the differential display. The significance of this method in investigation of differentially expressed genes is also discussed.

There are no comments on this title.

to post a comment.