Polycyclic aromatic hydrocarbon analysis in different matrices of the marine environment

Polycyclic aromatic hydrocarbons (PAHs)were determined in marine samples of various types, i.e. seawater, sediment and mussel homogenate samples. The samples were spiked with standard PAH mixtures in both polar (acetonitrile)and non-polar (i-octane)solvents, then extracted. Extraction from seawater was performed by liquid/liquid extraction to hexane (LLE)and with solid phase extraction (SPE)discs. The water samples were filtered and unfiltered seawater, and redistilled water for comparison. The discs with PAHs adsorbed from water samples, and also the sediment and mussel homogenate samples, were extracted with acetonitrile by sonication. PAHs in the disc extracts and from the LLE were cleaned-up using TLC and next determined by GC/MS/IT (with ion-trap)and HPLC-DAD/UV. The analytical procedures were verified with deuterated PAH standard mixtures. The large differences in PAH recoveries (from 12 to 86