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Prime genome editing in rice and wheat.

Tipo de material: TextoTextoSeries ; Nature Biotechnology, 38(5), p.582-585, 2020Trabajos contenidos:
  • Lin, Q
  • Zong, Y
  • Xue, C
  • Wang, S
  • Jin, S
  • Zhu, Z
  • Liu, D. R
Tema(s): Recursos en línea: Resumen: Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8 percent.
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Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8 percent.

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