1. A general introduction to and background of plant tissue culture: Past, current, and future aspects. 2. Micropropagation for multiplication of disease-free and genetically uniform sugarcane plantlets. 3. Role of plant tissue culture medium components. 4. The journey and new breakthroughs of plant growth regulators in tissue culture. 5. Plant tissue culture: Somatic embryogenesis and organogenesis. 6. Genetic transformation via plant tissue culture techniques: Current and future approaches. 7. Anther culture for haploid plant production. 8. Morpho-anatomical characterization of in vitro regenerated plants. 9. Plant tissue culture targeting germplasm conservation. 10. Somatic embryogenesis in medicinal plants. 11. Embryo rescue: A potential tool for improvement of economically important crops. 12. Strategies to overcome explant recalcitrance under in vitro conditions. 13. Improving crops through transgenic breeding-Technological advances and prospects. 14. Meristem culture: A potential technique for in vitro virus-free plants production in vegetatively propagated crops. 15. Plant tissue culture in tree species. 16.The role of plant tissue culture in pharmaceuticals and secondary metabolites production. 17. Tissue culture: A perpetual source for the conservation of medicinally important endangered plant species. 18. Phytochemical studies in the field of plant tissue culture. 19.

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