A Simple Spectrofluorometric Method for the Determination of Total Auxins in Mung Bean Sprouts.

Auxins play a very important role in plant development and growth. Herein a rapid and convenient method was developed for the quantification of total auxins including indole-3-acetic acid, indole-3-propionic acid and indole-3-butyric acid in mung bean sprouts by means of spectrofluorometry. Based on the same fluorescence properties of the compounds, a linear working curve was established between total concentrations of auxins and fluorescence intensities of their solutions. Under optimized conditions, correlation coefficient and limit of detection were found to be 0.9993 and 0.03 µg/mL, respectively, in linear range of 0.10-24 µg/mL. This method was successfully employed to the determination of total auxins after their extraction from mung bean sprouts, and the recovery was found to be in the range of 100-104percent with RSD below 5percen. This method has been proved to be simple, reproducible and accurate, also applicable for food samples without complicated apparatus and procedures.