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An improved HPLC assay for phosphatidylcholine hydroperoxides (PCOOH)in human plasma with synthetic PCOOH as internal standard

Tipo de material: TextoTextoSeries ; Journal of Chromatography B, 857(1), p.158-163, 2007Trabajos contenidos:
  • Hui, S
  • Chiba, H
  • Sakurai, T
  • Asakawa, C
  • Nagasaka, H
  • Murai, T
  • Ide, H
  • Ide, H
Tema(s): Recursos en línea: Resumen: Quantitative and qualitative analyses of 1-palmitoyl-2-linoleoyl-phosphatidylcholine monohydroperoxide [PC 16:0/18:2-OOH]and 1-stearoyl-2-linoleoyl-phosphatidylcholine monohydroperoxide [PC 18:0/18:2-OOH]in human plasma were improved by chemiluminescence HPLC usingsynthetic 1-stearoyl-2-erucoyl-phosphatidylcholine monohydroperoxide (PC 18:0/22:1-OOH)as internal standard. The calibration curves of synthetic PC 16:0/18:2-OOH and PC 18:0/18:2-OOH, obtained by their direct injections with the IS into the HPLC system, were linear throughout the calibration range (10-1000 pmol).Within-day and between-day coefficients of variation were below
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-10795 (Browse shelf(Opens below)) Available

Quantitative and qualitative analyses of 1-palmitoyl-2-linoleoyl-phosphatidylcholine monohydroperoxide [PC 16:0/18:2-OOH]and 1-stearoyl-2-linoleoyl-phosphatidylcholine monohydroperoxide [PC 18:0/18:2-OOH]in human plasma were improved by chemiluminescence HPLC usingsynthetic 1-stearoyl-2-erucoyl-phosphatidylcholine monohydroperoxide (PC 18:0/22:1-OOH)as internal standard. The calibration curves of synthetic PC 16:0/18:2-OOH and PC 18:0/18:2-OOH, obtained by their direct injections with the IS into the HPLC system, were linear throughout the calibration range (10-1000 pmol).Within-day and between-day coefficients of variation were below

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