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In vitro propagation of plant virus using different forms of plant tissue culture nd modes of culture operation

Tipo de material: TextoTextoSeries ; Journal of Biotechnology, 143(3), p.198-206, 2009Trabajos contenidos:
  • Shih, S.M.H
  • Doran, P.M
Tema(s): Recursos en línea: Resumen: Plant virus accumulation was investigated in vitro using three different forms of plant tissue culture. Suspended cells, hairy roots and shooty teratomas of Nicotiana benthamiana were infected with tobacco mosaic virus (TMV)using the same initial virus:biomass ratio. Viral infection did not affect tissue growth or morphology in any of the three culture systems. Average maximum virus concentrations in hairy roots and shooty teratomas were similar and about an order of magnitude higher than in suspended cells. Hairy roots were considered the preferred host because of their morphological stability in liquid medium and relative ease of culture. The average maximum virus concentration in the hairy roots was 0.82±0.14mgg-1 dry weight; viral coat protein represented a maximum of approximately 6
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Plant virus accumulation was investigated in vitro using three different forms of plant tissue culture. Suspended cells, hairy roots and shooty teratomas of Nicotiana benthamiana were infected with tobacco mosaic virus (TMV)using the same initial virus:biomass ratio. Viral infection did not affect tissue growth or morphology in any of the three culture systems. Average maximum virus concentrations in hairy roots and shooty teratomas were similar and about an order of magnitude higher than in suspended cells. Hairy roots were considered the preferred host because of their morphological stability in liquid medium and relative ease of culture. The average maximum virus concentration in the hairy roots was 0.82±0.14mgg-1 dry weight; viral coat protein represented a maximum of approximately 6

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