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Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

Tipo de material: TextoTextoSeries ; Nature Biotechnology, 35(5), p.441-443, 2017Trabajos contenidos:
  • Shimatani, Z
  • Kashojiya, S
  • Takayama, M
  • Terada, R
  • Arazoe, T
  • Ishii, H
  • Teramura, H
  • Teramura, H
  • Komatsu, H
  • Miura, K
  • Ezura, H
  • Nishida, K
  • Ariizumi, T
  • Kondo, A
Tema(s): Recursos en línea: Resumen: We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID)for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs)in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-16602 (Browse shelf(Opens below)) Available

We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID)for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs)in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.

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