Melatonin modifies the expression of the genes for nuclear- and plastidencoded chloroplast proteins in detached Arabidopsis leaves exposed to photooxidative stress

Melatonin, a potent regulator during plant development and stress responses, affects diverse plastid-related processes. However, its role in the regulation of plastid gene expression is largely unknown. In this study, exogenous melatonin was shown to reduce the negative influence of excess light by increasing the efficiency of the photosystems and rearranging the expression of chloroplast- and nuclear-encoded genes in detached Arabidopsis leaves. The positive effects of melatonin predominantly occurred at lower concentrations, while high doses had an inhibitory effect. The impact of melatonin was not straightforward. It mainly influenced the expression of the genes encoding the chloroplast transcription machinery and housekeeping genes involved in maintaining transcriptional activity and the functional state of chloroplasts. Despite the fact that melatonin treatment improved photosynthetic parameters, the levels of photosynthesis gene transcripts and photosynthetic proteins remained practically unaltered suggesting that melatonin impact on photosynthetic apparatus which would allow the balancing of chloroplast functions with stress responses is highly complicated.