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Tagging and localization of a phospholipase D gene in Coprinellus congregatus by restriction enzyme-mediated integration and pulsed-field gel electrophoresis por Series ; We have identified a phospholipase D gene (pld)fragment from a transformant generated by restriction enzyme-mediated integration in Coprinellus congregatus, which is a mushroom-forming basidiomycete. A fragment of pld of this fungus has been cloned from a transformant by gene tagging. The transformation vector has been inserted into pld and this has resulted in a decreased enzyme activity of the transformant compared with the wild strain. C. congregatus has seven chromosomes, the range of its genome size is 1.6-4.7 Mb and pld is located at chromosome 4 where the transformation vector has been inserted., 225(2), p.285-289, 2003
Tipo de material:  Texto; Formato:
electrónico
Disponibilidad: Ítems disponibles para préstamo: CICY (1)Colección, signatura topográfica: Ref1 B-6108.
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