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Enzymatic Synthesis and Purification of Aromatic Coenzyme A Esters

Tipo de material: TextoTextoSeries ; Analytical BioChemistry, 302(2), p.305-312, 2002Trabajos contenidos:
  • Beuerle, T
  • Pichersky, E
Tema(s): Recursos en línea: Resumen: Two recombinant His-tagged proteins, a plant 4-coumarate: coenzyme A ligase (EC 6.2.1.12)and a bacterial benzoate:coenzyme A ligase (EC 6.2.1.25), were expressed in Escherichia coli and purified in a single step using Ni-chelating chromatography. Purified enzymes were used to synthesize cinnamoyl-coenzyme A (CoA), p-coumaroyl-CoA, feruloyl-CoA, caffeoyl-CoA, and benzoyl-CoA. Conversions up to 95
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Two recombinant His-tagged proteins, a plant 4-coumarate: coenzyme A ligase (EC 6.2.1.12)and a bacterial benzoate:coenzyme A ligase (EC 6.2.1.25), were expressed in Escherichia coli and purified in a single step using Ni-chelating chromatography. Purified enzymes were used to synthesize cinnamoyl-coenzyme A (CoA), p-coumaroyl-CoA, feruloyl-CoA, caffeoyl-CoA, and benzoyl-CoA. Conversions up to 95

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