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245	1	0	_aIntegration of Simple Sequence Repeat (SSR)Markers Into a Molecular Linkage Map of Common Bean (Phaseolus vulgaris L.)
490	0		_vJournal of Heredity, 91(6), p.429-434, 2000
520	3		_aMicrosatellite or simple sequence repeat (SSR)markers have been successfully used for genomic mapping, DNA fingerprinting, and marker-assisted selection in many plant species. Here we report the first successful assignment of 15 SSR markers to the Phaseolus vulgaris molecular linkage map. A total of 37 SSR primer pairs were developed and tested for amplification and product-length polymorphism with BAT93 and Jalo EEP558, the parental lines of an F7 recombinant inbred (RI)population previously used for the construction of a common bean molecular linkage map. Sixteen of the SSRs polymorphic to the parental lines were analyzed for segregation and 15 of them were assigned to seven different linkage groups, indicating a widespread distribution throughout the bean genome. Map positions for genes coding for DNAJ-like protein, pathogenesis-related protein 3, plastid-located glutamine synthetase, endochitinase, sn-glycerol-3 phosphate acyltransferase, NADP-dependent malic enzyme, and protein kinase were determined for the first time. Addition of three SSR loci to linkage group B4 brought two separated smaller linkage groups together to form a larger linkage group. Analysis of allele segregation in the F7 RI population revealed that all 16 SSRs segregated in the expected 1:1 ratio. These SSR markers were stable and easy to assay by polymerase chain reaction (PCR). They should be useful markers for genetic mapping, genotype identification, and marker-assisted selection of common beans.
700	1	2	_aYu, K.
700	1	2	_aPark, S.J.
700	1	2	_aPoysa, V.
700	1	2	_aGepts, P.
856	4	0	_uhttps://drive.google.com/file/d/1TI1zUrJ8Mg_uH5adHFL7BSRxyeZvntDt/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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