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245	1	0	_aSequential solubilization of proteins precipitated with trichloroacetic acid in acetone from cultured Catharanthus roseus cells yields 52 more spots after two-dimensional electrophoresis
490	0		_vProteomics, 1(11), p.1345-1350, 2001
520	3		_aSample preparation is still the most critical step in two-dimensional gel electrophoresis (2-DE), and needs to be optimized for each type of sample. To analyze the proteome of the medicinal plant Catharanthus roseus, we developed and evaluated a sequential solubilization procedure for the solubilization of proteins after precipitation in trichloroacetic acid and acetone. The procedure includes solubilization with a conventional urea buffer followed by a stronger solubilizing buffer containing thiourea. The sequential solubilization of the precipitated proteins results in very different spot patterns following 2-DE. The number of protein spots which could be detected in both samples of the sequential solubilization was only about 10
700	1	2	_aJacobs, D
700	1	2	_avan Rijssen, M.S
700	1	2	_avan der Heijsen, R
700	1	2	_aVerpoorte, R.
856	4	0	_uhttps://drive.google.com/file/d/1IDhK4yTinMNx3FiDKc29t9lFkwSL20Ei/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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