000 02370nam a2200241Ia 4500
003 MX-MdCICY
005 20250625122508.0
040 _cCICY
090 _aB-5979
245 1 0 _aGenetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers
490 0 _vTheoretical and Applied Genetics, 107(2), p.271-282, 2003
520 3 _aCucurbita pepo is a highly polymorphic species. The cultivars can be grouped into eight morphotypes in two subspecies, ssp. pepo and ssp. ovifera. A collection of 69 accessions representative of the morphotypes and some unclassified types was used for analysing the morphological and molecular diversity of this species. This collection includes commercial cultivars and Spanish landraces, which represent the great diversification of types that have arisen in Europe after this species arrived from America. For the molecular variability studies, two PCR-based systems were employed, AFLP and SRAP, which preferentially amplify ORFs. Principal coordinates analysis and cluster analysis using the UPGMA method clearly separate the accessions into the two subspecies through the use of both markers. However, the gene diversity and the genetic identity values among morphotypes and subspecies varied between the two marker systems. The information given by SRAP markers was more concordant to the morphological variability and to the evolutionary history of the morphotypes than that of AFLP markers. In ssp. ovifera, the accessions of the different morphotypes were basically grouped according to the fruit colour. This may indicate different times of development and also the extent of breeding in the accessions used. This study has allowed identification of new types that can be employed for the development of new cultivars. The landraces of the spp. ovifera, used as ornamental in Europe, have proved to be of great interest for preserving the diversity of C. pepo.
650 1 4 _aCUCURBITA PEPO
650 1 4 _aGERMPLASM COLLECTION
650 1 4 _aAFLP MARKERS
650 1 4 _aSRAP MARKERS
700 1 2 _aFerriol, M.
700 1 2 _aPicó, B.
700 1 2 _aNuez, F.
856 4 0 _uhttps://drive.google.com/file/d/1FGsyp3xiclFW8OQRvb5hC5HpzjnuDHvS/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942 _2Loc
_cREF1
008 250602s9999 xx |||||s2 |||| ||und|d
999 _c40324
_d40324