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090			_aB-5985
245	1	0	_aTransgenic plants of coffea canephora from embryogenic callus via agrobacterium tumefaciens-mediated transformation
490	0		_vPlant Cell Reports, 19, p.106-110, 1999
520	3		_aAbstract Embryogenic calli were induced from leaf explants of coffee (Coffea canephora)on McCown's woody plant medium (WPM)supplemented with 5 7M N6-(2-isopentenyl)-adenosine (2-iP). These calli were co-cultured with Agrobacterium tumefaciens EHA101 harboring pIG121-Hm, containing #-glucuronidase (GUS), hygromycin phosphotransferase (HPT), and neomycin phosphotransferase II genes. Selection of putative transgenic callus was performed by gradual increase in hygromycin concentration (5, 50, 100 mg/l). The embryogenic calli surviving on medium containing 100 mg/l hygromycin showed a strong GUS-positive reaction with X-Gluc solution. Somatic embryos were formed from these putative transgenic calli and germinated on WPM medium with 5 7M 2-iP. Regenerated small plantlets with shoots and roots were transferred to medium containing both 100 mg/l hygromycin and 100 mg/l kanamycin for final selection of transgenic plants. The selected plantlets exhibited strong GUS activity in leaves and roots as indicated by a deep blue color. GUS and HPT genes were confirmed to be stably integrated into the genome of the coffee plants by the polymerase chain reaction.
650	1	4	_aCOFFEA CANEPHORA
650	1	4	_aAGROBACTERIUM TUMEFACIENS
650	1	4	_aTRANSFORMATION
700	1	2	_aHatanaka, T.
700	1	2	_aChoi, Y.E.
700	1	2	_aKusano, T.
700	1	2	_aSano, H.
856	4	0	_uhttps://drive.google.com/file/d/1oJ4ThleYakSaic7u2MbLHjcc-Wly_VSf/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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