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245	1	0	_aGenetically modified coffee plants expressing the Bacillus thuringiensis cry1Ac gene for resistance to leaf miner
490	0		_vPlant Cell Reports, 19, p.382-389, 2000
520	3		_aAbstract A synthetic version of the cry1Ac gene of Bacillus thuringiensis has been used for the transformation of coffee species (Coffea canephora and C. arabica)to confer resistance to an important pest, the coffee leaf miner (Perileucoptera coffeella and other Leucoptera spp). Somatic embryos were co-cultivated with the LBA4404 strain of Agrobacterium tumefaciens containing the cry1Ac gene. More than 100 transformed plants from independent transformation events were obtained for each coffee genotype. The integration and expression of the cry1Ac gene was studied, and effective resistance of transgenic plants against leaf miner was verified in bioassays with the insects. These plants could represent a good opportunity to analyse the impact of genetic engineering of perennial crops for sustainable resistance to an obligate endocarpic pest using a B. thuringiensis insecticidal protein.
650	1	4	_aCRY 1AC GENE
650	1	4	_aCOFFEA SPP
650	1	4	_aGENETIC TRANSFOMATION
650	1	4	_aPERILEUCOPTERA COFFEELLA
650	1	4	_aLEUCOPTERA SPP
700	1	2	_aLeroy, T.
700	1	2	_aHenry, A. -M.
700	1	2	_aRoyer, M.
700	1	2	_aAltosaar, I.
700	1	2	_aFrutos, R.
700	1	2	_aDuris, V
700	1	2	_aPhilippe, R.
856	4	0	_uhttps://drive.google.com/file/d/1ft-AMzC9YeMZvl1SCMEM_cwDvbDhB9cQ/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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