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245 1 0 _aFusicoccin induces in plant cells a programmed cell death showing apoptotic features
490 0 _vProtoplasma, 222, p.113-116, 2003
520 3 _aProgrammed cell death plays a pivotal role in several developmental processes of plants and it is involved in the response to environmental stresses and in the defense mechanisms against pathogen attack. It has not yet been defined which part of the death signalling mechanism and which molecules involved in programmed cell death are common to animals and plants. In this paper we show that fusicoccin, a well-known phytotoxin, induces a strong acceleration in the appearance of Evans Blue-stainable (dead)cells in sycamore (Acer pseudoplatanus L.)cultures. This fusicoccin-induced cell death shows aspects common to the form of animal programmed cell death termed apoptosis: i.e., cell shrinkage, changes in nucleus morphology, increase in DNA fragmentation detectable by a specific immunological reaction, and presence of oligonucleosomal-size fragments (laddering)in DNA gel electrophoresis. Since fusicoccin has a well-identified molecular target, the plasma membrane H+-ATPase, and thoroughly investigated physiological effects, this toxin appears to be a useful tool to study the transduction of death signals leading to programmed cell death in plants.
650 1 4 _aAPOPTOSIS
650 1 4 _aDNA FRAGMENTATION
650 1 4 _aFUSICOCCIN
650 1 4 _aPLANT CELL CULTURE
650 1 4 _aPROGRAMMED CELL DEATH
650 1 4 _aTERMINAL DEOXYNUCLEOTIDYL TRANSFERASE-MEDIATED DUTP NICK END LABELLING
700 1 2 _aMalerba, M.
700 1 2 _aCerana, R.
700 1 2 _aCrosti, P.
856 4 0 _uhttps://drive.google.com/file/d/1-vB61uvJJRcsbN91MsydisTq3b2-LxVR/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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