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090 _aB-6047
245 1 0 _aCharacteristics of growth and tropane alkaloid synthesis in Atropa belladonna roots transformed by Agrobacterium rhizogenes
490 0 _vJournal of Biotechnology, 16(3-4), p.171-186, 1990
520 3 _aGrowth of Atropa belladonna hairy roots in shake flasks followed first-order kinetics. The specific growth rate was 0.28 d-1, corresponding to a doubling time of 60 h. In airlift reactors, first order kinetics were not observed due to nutrient limitation. Synthesis of tropane alkaloids was growth-associated; no atropine was released into the medium. Sugar concentrations were analyzed during hairy-root culture for calculation of atropine yield. Atropine yields from substrate were 1.4 mg g-1 in shake flasks and 0.46 mg g-1 in airlift reactors. Although levels of atropine in reactor-grown roots (0.37
650 1 4 _aAGROBACTERIUM RHIZOGENES
650 1 4 _aHAIRY ROOT CULTURE
650 1 4 _aBIOCHEMICAL ENGINEERING
650 1 4 _aATROPA BELLADONNA
650 1 4 _aATROPINE
650 1 4 _aTROPANE ALKALOIDS
700 1 2 _aSharp, J.M.
700 1 2 _aDoran, P.M.
856 4 0 _uhttps://drive.google.com/file/d/1oq7XS7IPpo2n7VefLSUOopMJTgfPHqhE/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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