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040			_cCICY
090			_aB-6049
245	1	0	_aElectrophoretic Karyotype of the Obligate Biotrophic Parasite Plasmodiophora brassicae Wor.
490	0		_vJournal of PhytoPathology, 149(6), p.313-318, 2001
520	3		_aClassical genetic analysis is not possible with the protist Plasmodiophora brassicae due to the intracellular life of this obligate biotrophic parasite. An electrophoretic karyotype has been obtained using contour-clamped homogeneous electric field gel electrophoresis to facilitate gene mapping of P. brassicae. Using two different separation conditions 16 chromosomal bands of P. brassicae were distinguished ranging in approximate size from 2.2 Mb to 680 kb. According to this determination of chromosome number and size, the total genome size of P. brassicae was estimated to be 20.3 Mb. The chromosomal bands were further designated by their hybridization pattern with repetitive elements of P. brassicae. The repetitive element H4 (1800 bp)hybridized with 14 chromosomal bands, but the sequence of H4 showed no homology to known centromere or telomere structures and revealed no repetitive motifs.
650	1	4	_aCLUBROOT
650	1	4	_aCHROMOSOME NUMBER
700	1	2	_aGraf, H.
700	1	2	_aSokolowski, F.
700	1	2	_aKlewer, A.
700	1	2	_aDiederichsen E.
700	1	2	_aLuerßen, H.
700	1	2	_aSiemens J.
856	4	0	_uhttps://drive.google.com/file/d/1ePqRnwLbP6XfPh5R7_n1Tk8lgA3xfHvH/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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