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090			_aB-6110
245	1	0	_aA user's guide to restriction enzyme-mediated integration in Dictyostelium
490	0		_vJournal of Muscle Research and Cell Motility, 23 (7-8), p.597-604, 2002
520	3		_aRestriction enzyme-mediated integration (REMI)has been used to study a number of cellular and developmental processes in Dictyostelium discoideum. In this paper we review the basics of this powerful method of introducing random mutations in Dictyostelium. Here we discuss several mutation screens that have been devised and some of the genes that have been discovered through this approach to mutagenesis. Included in this discussion is how one goes about isolating a gene that has been disrupted by REMI, and how one confirms that this disruption is actually responsible for the observed phenotype. Finally, we describe how REMI can be used as an effective teaching tool in undergraduate cell biology laboratory courses.
700	1	2	_aGuerin, N.A.
700	1	2	_aLarochelle, D.A.
856	4	0	_uhttps://drive.google.com/file/d/1wLB39I-UVrx22K02_m_z-yvaASueRt3O/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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