000			01604nam a2200301Ia 4500
003			MX-MdCICY
005			20250625124639.0
040			_cCICY
090			_aB-6270
245	1	0	_aHighly conserved sequences flank avirulence genes: isolation of novel avirulence genes from Pseudomonas syringae pv. pisi
490	0		_vMicroBiology, 147(5), p.1171-1182, 2001
520	3		_aDNA sequences flanking two avr genes (avrPpiA1 and avrPpiB1)from Pseudomonas syringae pv. pisi show a high degree of similarity. Specific primers designed from the conserved regions were used in PCR amplifications with all P. syringae pv. pisi races. As well as amplifying the expected avrPpiA- and avrPpiB-containing fragments, two additional fragments were amplified: one contained a single open reading frame (ORF1)and was found in races of genomic group II (2, 3A, 4A and 6); the second fragment contained two open reading frames (ORF2 and ORF3), separated by 658 nt, and was detected in all races. All three ORFs had G+C ratios (46·9-48 mol
650	1	4	_aPSEUDOMONAS SYRINGAE
650	1	4	_aCONSERVED DNA SEQUENCES
650	1	4	_aAVR
650	1	4	_aPLASMID
650	1	4	_aRULAB
700	1	2	_aArnold, D.L.
700	1	2	_aJackson, R.W.
700	1	2	_aFillingham, A.J.
700	1	2	_aGoss, S.C.
700	1	2	_aTaylor, J.D.
700	1	2	_aMansfield, J.W.
700	1	2	_aVivian, A.
856	4	0	_uhttps://drive.google.com/file/d/19OnoeppNb_blz9CsinjifrTYkdyXH0bL/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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999			_c40615 _d40615