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090			_aB-6295
245	1	0	_aDevelopment of a transformation system for Mycosphaerella pathogens of banana: a tool for the study of host/pathogen interactions
490	0		_vFEMS MicroBiology Letters, 195(1), p.9-15, 2001
520	3		_aA genetic transformation system has been developed for three Mycosphaerella pathogens of banana and plantain (Musa spp.). Mycosphaerella fijiensis and Mycosphaerella musicola, the causal agents of black and yellow Sigatoka, respectively, and Mycosphaerella eumusae, which causes Septoria leaf spot of banana, were transformed with a construct carrying a synthetic gene encoding green fluorescent protein (GFP). Most single-spored transformants that expressed GFP constitutively were mitotically stable in the absence of selection for hygromycin B resistance. Transformants of all three species were pathogenic on the susceptible banana cultivar Grand Nain, and growth in planta was comparable to wild-type strains. GFP expression by transformants allowed us to observe extensive fungal growth within leaf tissue that eventually turned necrotic, at which point the fungi grew saprophytically on the dead tissue. Leaf chlorosis and necrosis were often observed in advance of saprophytic growth of the mycelium on necrotic tissue, which supports previous reports suggesting secretion of a phytotoxin.
650	1	4	_aFUNGAL TRANSFORMATION
650	1	4	_aPLANT DISEASE
650	1	4	_aGREEN FLUORESCENT PROTEIN
650	1	4	_aMUSA
700	1	2	_aBalint-Kurti, P.J.
700	1	2	_aMay, G.D.
700	1	2	_aChurchill, A.C.L.
856	4	0	_uhttps://drive.google.com/file/d/11JfVXMneq2Hy2QXEA6TEYufuY-nPJyzu/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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