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245	1	0	_aREMI-induced mutants ofMycosphaerella zeae-maydislacking the polyketide PM-toxin are deficient in pathogenesis to corn
490	0		_vPhysiological and Molecular Plant Pathology, 52(1), p.53-66, 1998
520	3		_aThe polyketide PM-toxin, produced byMycosphaerella zeae-maydis, is specifically active against corn containing Texas male sterile (T)cytoplasm, as is the fungus itself. To determine if PM-toxin is required for disease development,Tox-mutants were generated using the restriction enzyme-mediated integration (REMI)strategy, which is designed to tag the mutations it creates. As a mutagenic procedure, REMI was highly efficient, yielding five stable PM-toxin-deficient mutants among 504 transformants recovered. AllTox-mutants lost the ability to cause disease on T-cytoplasm corn, establishing a role for PM-toxin in pathogenesis. Examination of genomic DNAs from theTox-mutants revealed one that carried the transformation vector at multiple insertion sites and two that had no apparent ORFs at single vector insertion sites; three sustained deletions.
700	1	2	_aYun, S.-H.
700	1	2	_aTurgeon, B.G.
700	1	2	_aYoder, O.C.
856	4	0	_uhttps://drive.google.com/file/d/1RuHyuwPt8LtxPDX5v5xeHKm7k5eQeTyw/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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