000			01870nam a2200193Ia 4500
003			MX-MdCICY
005			20250625124643.0
040			_cCICY
090			_aB-6465
245	1	0	_aBarbiturate Induced Benzophenanthridine Alkaloid Formation Proceeds by Gene Transcript Accumulation in the California Poppy
490	0		_vBioChemical and Biophysical Research Communications, 241(2), p.606-610, 1997
520	3		_aFour barbiturates, barbituric acid, butethal, phenobarbital, and 2-thiobarbituric acid, of fourteen tested were found to induce accumulation of benzophenanthridine alkaloids in cell suspension cultures of the California poppyEschscholzia californica.When the plant cell suspension cultures were treated with 1 mM barbiturate, alkaloids accumulated to 100 mg/l within four days. This is a level comparable to that achieved with 300 M concentration of the established secondary metabolite inducer methyl jasmonate. In contrast to methyl jasmonate, barbituric acid, and 2-thiobarbituric acid, butethal and phenobarbital treatment resulted in a different alkaloid profile, suggesting that only select cytochrome P-450 genes were activated by these latter two barbiturates. RNA gel blot analysis of barbiturate induced cell cultures confirmed that transcripts of at least two benzophenanthridine alkaloid biosynthetic genescyp80b1(encoding the cytochrome P-450-dependent monooxygenase (S)-N-methylcoclaurine 3'-hydroxylase)andbbe1(encoding the covalently flavinylated berberine bridge enzyme)increased up to 5- to 7-fold over control values.
700	1	2	_aHaider, G.
700	1	2	_aKislinger, T.
700	1	2	_aKutchan, T.M.
856	4	0	_uhttps://drive.google.com/file/d/1Wx3ixaU3uA-pMoZVmmczCUVvZoCtAb0Q/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c40808 _d40808