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245	1	0	_aA Large-Insert (130 kbp)Bacterial Artificial Chromosome Library of the Rice Blast FungusMagnaporthe grisea:Genome Analysis, Contig Assembly, and Gene Cloning
490	0		_vFungal Genetics and Biology, 21(3), p.337-347, 1997
520	3		_aMagnaporthe grisea(Hebert)Barr causes rice blast, one of the most devastating diseases of rice (Oryza sativa)worldwide. This fungus is an ideal organism for studying a number of aspects of plant-pathogen interactions, including infection-related morphogenesis, avirulence, and pathogen evolution. To facilitateM. griseagenome analysis, physical mapping, and positional cloning, we have constructed a bacterial artificial chromosome (BAC)library from the rice infecting strain 70-15. A new method was developed for separation of partially digested large-molecular-weight DNA fragments that facilitated library construction with large inserts. The library contains 9216 clones, with an average insert size of 130 kbp (>25 genome equivalents)stored in 384-well microtiter plates that can be double spotted robotically on to a single nylon membrane. Several unlinked single-copy DNA probes were used to screen 4608 clones in the library and an average of 13 (minimum of 6)overlapping BAC clones was found in each case. Hybridization of total genomic DNA to the library and analysis of individual clones indicated that ~26
700	1	2	_aZhu, H.
700	1	2	_aChoi, S.
700	1	2	_aJohnston, A.K.
700	1	2	_aWing, R.A.
700	1	2	_aDean, R.A.
856	4	0	_uhttps://drive.google.com/file/d/1_irRuNWngWeTMJ2wrEzPtSz6Sike1aQD/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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