000			01963nam a2200217Ia 4500
003			MX-MdCICY
005			20250625124643.0
040			_cCICY
090			_aB-6477
245	1	0	_aGeminivirus Resistance in Transgenic Tobacco Expressing Mutated BC1 Protein.
490	0		_vMolecular Plant-Microbe Interactions, 10(7), p.617-623, 1997
520	3		_aTobacco explants were transformed by Agrobacterium-mediated transformation with sense and antisense constructs of the movement protein genes (BC1 and BV1)of tomato mottle geminivirus (TMoV). Transgenic plants were tested for virus resistance either by exposure to viruliferous whiteflies carrying TMoV or cabbage leaf curl geminivirus (CabLCV)for a 72-h inoculation period or by continuous exposure to TMoV during the life of the plants. The transgenic lines were scored for disease symptoms, and virus replication and distribution were determined by enzyme-linked immunosorbent assay and dot blot hybridizations. Transgenic plants which expressed a mutated form (identified in a previous study)of the BC1 gene showed TMoV and CabLCV resistance. Three resistant phenotypes were observed: a delay in symptom development, a recovery from early symptoms, and an absence of virus symptoms at all stages. Geminivirus was detected in inoculated leaves but was not readily detected in leaves beyond the inoculation sites in the highly resistant plants. The geminivirus resistance conferred by expression of the spontaneously mutated BC1 gene (detected after transformation)in transgenic tobacco may involve transdominant negative interference.
700	1	2	_aDuan, Y.P.
700	1	2	_aPowell, C.A.
700	1	2	_aWebb, S.E.
700	1	2	_aPurcifull, D.E.
700	1	2	_aHiebert, E.
856	4	0	_uhttps://drive.google.com/file/d/16sv8NtEAnlCloiYjN-fcFNZq6LzTV9Z-/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c40820 _d40820