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245	1	0	_aO2- from elicitor-induced oxidative burst is necessary for triggering phenylalanine ammonia-lyase activation and catharanthine synthesis in Catharanthus roseus cell cultures
490	0		_vEnzyme and Microbial Technology, 36(2-3), p.280-284, 2005
520	3		_aElicitor, derived from the cell walls of Aspergillus niger, induced rapid generation of reactive oxygen intermediates (ROI), including superoxide anion (O2-)and hydrogen peroxide (H2O2), sequentially followed by phenylalanine ammonia-lyase (PAL)activation and catharanthine biosynthesis in Catharanthus roseus suspension cells. The elicitor-induced PAL activation and catharanthine biosynthesis were blocked by NAD(P)H oxidase inhibitor, diphenylene iodonium (DPI). O2- generated by the reaction of xanthine/xanthine oxidase (X/XO)triggered PAL activation and catharanthine biosynthesis of C. roseus cells in the absence of elicitor and reversed the inhibitory effect of DPI on elicitor-induced PAL activation and catharanthine biosynthesis. External application of H2O2 and catalase had no effect on PAL activity and catharanthine contents of C. roseus cells. The results demonstrated a causal relationship between elicitor-induced oxidative burst and PAL activation in C. roseus suspension cells and suggested a sequence of signaling events from ROI production to PAL activation and catharanthine synthesis. Within this sequence, O2- rather than H2O2 appeared to trigger the subsequent reactions
650	1	4	_aSUPEROXIDE ANIONS
650	1	4	_aH2O2
650	1	4	_aELICITOR
650	1	4	_aCATHARANTHUS ROSEUS CELLS
650	1	4	_aCATHARANTHINE
650	1	4	_aPAL
700	1	2	_aXu, M.
700	1	2	_aDong, J.
856	4	0	_uhttps://drive.google.com/file/d/19ao8nfc3SxD76rFDSNqfKRX5N4_v0HOr/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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