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245	1	0	_aAnalysis And Localization of the Water-Deficit Stress-Induced Gene (lp3)
490	0		_vJournal of Plant Growth Regulation , 21(4), p.469-478, 2003
520	3		_aLP3 is a water-deficit-induced protein, which is highly homologous to ASR (ABA, stress and ripening)proteins. Homology was found in the C-terminal region of the putative LP3 protein while lower homologies were found in the N-terminal region. The goal of this study was to investigate the function of the LP3 protein and the mechanism of the lp3 promoter in response to water-deficit stress (WDS)and other stresses. In regenerated transgenic tobacco (T0), expression of -glucuronidase (GUS)from the lp3 promoter-GUS construct was observed in polyethylene glycol (PEG), abscisic acid (ABA), methyl-jasmonate (MeJa), and fluridone (Flu)treatments. GUS expression was not observed following gibberellin (GA3), 2-methyl-4-dichlorophenoxy acetic acid (2,4-D), silver nitrate, or ethephon (ethylene releasing agent)treatments. Germinated T1 seedlings containing the lp3 promoter-GUS construct exhibited GUS activity up to 40 days postgermination. Expression could be restored when 5-azacytidine was included in the culture media, indicative of a developmentally regulated silencing mechanism involving methylation. In transgenic tobacco, the LP3 protein localized in the cell nucleus was induced by WDS and appeared to be developmentally regulated.
650	1	4	_aTOBACCO
650	1	4	_aWATER-DEFICIT STRESS (WDS)
650	1	4	_aABSCISIC ACID (ABA)
650	1	4	_aPOLYETHYLENE GLYCOL (PEG)
650	1	4	_aFLURIDONE
650	1	4	_aLP3 GENE
650	1	4	_aMETHYLATION
700	1	2	_aWang, J. T.
700	1	2	_aGould, J. H.
700	1	2	_aPadmanabhan, V.
700	1	2	_aNewton, R. J.
856	4	0	_uhttps://drive.google.com/file/d/1W5XtGOm3Y7Ngke5Ym1Hr6Nsp5GJZk4qz/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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