000			01661nam a2200265Ia 4500
003			MX-MdCICY
005			20250625124649.0
040			_cCICY
090			_aB-6784
245	1	0	_aCloning and heterologous expression of a cDNA encoding l-deoxy-D-xylulose-5-phosphate reductoisomerase of Arabidopsis thaliana1
490	0		_vFEBS Letters, 455, p.140-144, 1999
520	3		_aVarious plant isoprenoids are synthesized via the nonmevalonate pathway of isopentenyl diphosphate formation. In this pathway, 1-deoxy-D-xylulose 5-phosphate (DOXP), the first intermediate, is transformed to 2-C-methyl-D-erythritol 4-phosphate (MEP)by an enzyme which was recently cloned from Escherichia coli. In order to find a plant homologue of this 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR)we cloned a cDNA fragment from Arabidopsis thaliana which has high homology to the E. coli DXR. By expression of this fragment in E. coli we could demonstrate that it encodes a protein which transforms DOXP to MEP. The antibiotic fosmidomycin pecifically inhibits this DXR enzyme activity.
650	1	4	_a1-DEOXY-D-XYLULOSE
650	1	4	_a1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE
650	1	4	_a1-DEOXY-D-XYLULOSE 5-PHOSPHATE PATHWAY
650	1	4	_aISOPRENOID BIOSYNTHESIS
650	1	4	_a2-C-METHYL-D-ERYTHRITOL
700	1	2	_aSchwender, J.
700	1	2	_aMuller, Ch.
700	1	2	_aZeidler, J.
700	1	2	_aLichtenthaler, H.K.
856	4	0	_uhttps://drive.google.com/file/d/17fLBAMxYhkDtTXhu0XupuduP3K_OKVMl/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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