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090			_aB-6871
245	1	0	_aDeveloping methods and strains for genetic studies in the Saccharomyces bayanus var. uvarum species
490	0		_vYeast, 21(14), p.1195-1203, 2004
520	3		_ainsight into complex biological processes. The study of closely related yeast species may be critical for understanding the molecular mechanism of evolution. Among those species, S. bayanus var. uvarum could be particularly pertinent because of the availability of its genome sequence. However, to date, in that species genetic studies are problematical due to the lack of standard strains collection and genetic methods. Here, we have developed heterothallic S. bayanus var. uvarum strains and obtained stable haploid strains. We further used UV-induced mutation and gene disruption to create a collection of auxotrophic derivatives. Finally, we have elaborated or improved methods to cultivate cells, obtain zygotes and spores and to transform this species. All these tools can now be used by the scientific community to study the biology of this species.
650	1	4	_aSACCHAROMYCES
650	1	4	_aSACCHAROMYCES BAYANUS VAR. UVARUM
650	1	4	_aGENETIC TOOLS
700	1	2	_aTalarek, N.
700	1	2	_aLouis, E.J.
700	1	2	_aCullin, Ch.
700	1	2	_aAigle, M.
856	4	0	_uhttps://drive.google.com/file/d/1OUQ8xLqQOSKp7mtWLBePtKLw-lAcnP_z/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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