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090			_aB-6921
245	1	0	_aConfocal Direct Imaging Raman Microscope: Design and Applications in Biology
490	0		_vApplied Spectroscopy, 52(3), p.348-355, 1998
520	3		_aA confocal direct imaging Raman microscope (CDIRM)based on two synchronized scanning mirrors, a monochromator, and two charge-coupled device (CCD)cameras has been developed. With this system it is possible to make both Raman spectra of a small measurement volume and images of a larger sample area in one speci® c Raman band. The spatial resolution of the system was determined for two limiting situations: a small sphere and a thin layer. The image of a 0.282 m m sphere appeared to have a full width at half-maximum (FWHM)of 1.2 m m in the axial and 0.37 m m in the lateral direction, whereas the image of a 275 nm layer showed an FWHM of 1.4 m m in the axial direction . Confocal Raman images were made of the DNA and protein distribution in polytene chromosomes with a relatively weak Raman signal [0.1 photons/(second ´pixel)]. Further, a three-dimensional Raman image of the drug distribution in a hthalocyanine-incubated ® xed cell is presented. These examples show that the CDIRM can be used to image samples with a weak Raman signal and that three-dimensional images of the distribution of speci® c molecules in a sample can be made
650	1	4	_aRAMAN
650	1	4	_aMICROSCOPY
650	1	4	_aSPECTROSCOPY
650	1	4	_aCONFOCAL
650	1	4	_aIMAGING
700	1	2	_aSijtsema, N.M.
700	1	2	_aWouters, S.D.
700	1	2	_aDe Grauw, C.J.
700	1	2	_aOtto, C.
700	1	2	_aGreve, J.
856	4	0	_uhttps://drive.google.com/file/d/1HxzYuPKZWKOIuFXQolb3bxflnZ2aH8-4/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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