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090			_aB-6972
245	1	0	_aA spectrophotometric assay of gamma-glutamylcysteine synthetase and glutathione synthetase in crude extracts from tissues and cultured mammalian cells.
490	0		_vChemico-Biological Interactions, 140(1), p.49-65, 2002
520	3		_aAn assay of gamma-glutamylcysteine synthetase (gamma-GCS)and glutathione synthetase (GS)in crude extracts of cultured cells and tissues is described. It represents a novel combination of known methods, and is based on the formation of glutathione (GSH)from cysteine, glutamate and glycine in the presence of rat kidney GS for the assay of gamma-GCS, or from gamma-glutamylcysteine and glycine for the assay of GS. GSH is then quantified by the Tietze recycling method. Assay mixtures contain the gamma-glutamyl transpeptidase (GGT)inhibitor acivicin in order to prevent the degradation of gamma-glutamylcysteine and of the accumulating GSH, and dithiothreitol in order to prevent the oxidation of cysteine and gamma-glutamylcysteine. gamma-GCS and GS levels determined by this method are comparable to those determined by others. The method is suitable for the rapid determination of gamma-GCS GS in GGT-containing tissues and for the studies of induction of gamma-GCS and GS in tissue cultures.
700	1	2	_aVolohonsky, G.
700	1	2	_aTuby, C.N.
700	1	2	_aPorat, N.
700	1	2	_aWellman-Rousseau, M.
700	1	2	_aVisvikis, A.
700	1	2	_aLeroy, P.
700	1	2	_aRashi, S.
700	1	2	_aRashi, S.
700	1	2	_aStark, A.A.
856	4	0	_uhttps://drive.google.com/file/d/1SYrWQWxVj5Vw1rY8qhvDkA2z9yCyWmQo/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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