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245 1 0 _aIsolation of a new dual-functional ca¡eine synthase gene encoding an enzyme for the conversion of 7-methylxanthine to ca¡eine from coffee (Coffea arabica L.)1
490 0 _vFebs Letters, 534, p.75-81, 2003
520 3 _aIn co¡ee and tea plants, ca¡eine is synthesized from xanthosine via a pathway that includes three methylation steps. We report the isolation of a bifunctional co¡ee ca¡eine synthase (CCS1)clone from co¡ee endosperm by reverse transcriptionpolymeras chain reaction (RT-PCR)and rapid ampli¢cation of cDNA ends (RACE)technique using previously reported sequence information for theobromine synthases (CTSs). The predicted amino acid sequences of CCS1are more than 80
650 1 4 _aCO¡EE
650 1 4 _aCA¡EINE
650 1 4 _aN-METHYLTRANSFERASE
650 1 4 _a7-METHYLXANTHINE
650 1 4 _aS-ADENOSYL-L-METHIONINE
650 1 4 _aTHEOBROMINE
700 1 2 _aMizunoa, K.
700 1 2 _aOkuda, A.
700 1 2 _aKato, M.
700 1 2 _aYoneyama, N.
700 1 2 _aTanaka, H.
700 1 2 _aAshihara, H.
700 1 2 _aFujimura, T
856 4 0 _uhttps://drive.google.com/file/d/1y6v-JV0a1C_uMO09hd04PBhHc4G-DIw_/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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