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090			_aB-7121
245	1	0	_aPolymorphism of PCR-based markers targeting exons, introns, promoter regions, and SSRs in maize and introns and repeat sequences in oat1
490	0		_vGenome, 44(6), p.1065-1076, 2001
520	3		_aSequence databases could be efficiently exploited for development of DNA markers if it were known which gene regions reveal the most polymorphism when amplified by PCR. We developed PCR primer pairs that target specific regions of previously sequenced genes from Avena and Zea species. Primers were targeted to amplify 40 introns, 24 exons, and 23 promoter regions within 54 maize genes. We surveyed 48 maize inbred lines (previously assayed for simple-sequence repeat (SSR)polymorphism)for implification-product polymorphism. We also developed primers to target 14 SSRs and 12 introns within 18 Avena genes, and surveyed 22 hexaploid oat cultivars and 2 diploid Avena species for amplification-product polymorphism. In maize, 67
700	1	2	_aHolland, J.B.
700	1	2	_aHelland, S.J.
700	1	2	_aSharopova, N.
700	1	2	_aRhyne, D.C.
856	4	0	_uhttps://drive.google.com/file/d/1xGkDAcDsdWYhEAohEXAwU6UYkzOqKEhg/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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