000			01667nam a2200193Ia 4500
003			MX-MdCICY
005			20250625124655.0
040			_cCICY
090			_aB-7122
245	1	0	_aGenotyping single nucleotide polymorphisms in barley by tetra-primer ARMS-PCR
490	0		_vGenome, 47(2), p.414-420, 2004
520	3		_aSingle nucleotide polymorphisms (SNPs)are the most abundant form of DNA polymorphism. These polymorphisms can be used in plants as simple genetic markers for many breeding applications, for population studies, and for germplasm fingerprinting. The great increase in the available DNA sequences in the databases has made it possible to identify SNPs by "database mining", and the single most important factor preventing their widespread use appears to be the genotyping cost. Many genotyping platforms rely on the use of sophisticated, automated equipment coupled to costly chemistry and detection systems. A simple and economical method involving a single PCR is reported here for barley SNP genotyping. Using the tetra-primer ARMS-PCR procedure, we have been able to assay unambiguously five SNPs in a set of 132 varieties of cultivated barley. The results show the reliability of this technique and its potential for use in low- to moderate-throughput situations; the association of agronomically important traits is discussed.
700	1	2	_aChiapparino, E.
700	1	2	_aLee, D.
700	1	2	_aDonini, P.
856	4	0	_uhttps://drive.google.com/file/d/1rER1VrDZ6sGl3vnaMmQ22SYVK5XUmfbF/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
942			_2Loc _cREF1
008			250602s9999 xx |||||s2 |||| ||und|d
999			_c41453 _d41453