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090			_aB-7158
245	1	0	_aHigh throughput immuno-screening of cDNA expression libraries produced by in vitro recombination; exploring the Plasmodium falciparum proteome
490	0		_vMolecular & BioChemical Parasitology, 133(2), p.267-274, 2004
520	3		_aImproved Plasmodium falciparum cDNA expression libraries were constructed by combining mRNA oligo-capping with in vitro recombination and directional cloning of cDNA inserts into a plasmid vector that expresses sequences as thioredoxin fusion proteins. A novel procedure has also been developed for the rapid identification of seropositive clones on high-density filters, using direct labelling of P. falciparum immune immunoglobulin with fluorescein isothiocynate (FITC). This approach combines the advantages of recombination-assisted cDNA cloning with high throughput, non-radioactive serological screening of expression libraries. Production of replicate colony matrices allows the identification of antigens recognised by different pools with different specificities from residents of a malaria endemic region. Analyses ofDNAsequences derived from sero-reactive colonies indicate that this is an effective method for producing recombinant proteins that react with antibodies from malaria-exposed individuals. This approach permits the systematic construction of a database of antigenic proteins recognised by sera from malaria-exposed individuals.
650	1	4	_aPLASMODIUM FALCIPARUM
650	1	4	_aCDNA
650	1	4	_aIN VITRO RECOMBINATION
650	1	4	_aPROTEIN MACRO-ARRAY
700	1	2	_aKordai Sowa, M.P.
700	1	2	_aSharling, L.
700	1	2	_aHumphreys, G.
700	1	2	_aCavanagh, D.R.
700	1	2	_aGregory, W.F.
700	1	2	_aFenn, K.
700	1	2	_aCreasey, A.M.
700	1	2	_aCreasey, A.M.
856	4	0	_uhttps://drive.google.com/file/d/16z7CnuDjltYaDM9Y2rm17rgEvbwuFLUG/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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