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090			_aB-7212
245	1	0	_aScreening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae
490	0		_vMol. Gen. Genet., 258(1-2), p.53-59, 1998
520	3		_aOpen reading frames in the genome of Saccharomyces cerevisiae were screened for potential glycosylphosphatidylinositol (GPI)-attached proteins. The identi®cation of putative GPI-attached proteins was based on three criteria: the presence of a GPI-attach-ment signal sequence, a signal sequence for secretion and a serine- or threonine-rich sequence. In all, 53 ORFs met these three criteria and 38 were further analyzed as follows. The sequence encoding the 40 C- terminal amino acids of each was fused with the struc- tural gene for a reporter protein consisting of a secretion signal, a-galactosidase and a hemagglutinin (HA)epitope, and examined for the ability to become incorpo- rated into the cell wall. On this basis, 14 of fusion proteins were classi®ed as GPI-dependent cell wall proteins because cells expressing these fusion proteins: (i)had high levels of a-galactosidase activity on their surface; (ii)released signi®cant amounts of the fusion proteins from the membrane on treatment with phosphatidylinositol-speci®c phospholipase C (PI-PLC); and (iii)released fusion proteins from the cell wall following treatment with laminarinase. Of the 14 identi®ed putative GPI-dependent cell wall proteins, 12 had novel ORFs adjacent to their GPI-attachment signal sequence. Amino acid sequence alignment of the C-terminal sequences of the 12 ORFs, together with those of known cell wall proteins, reveals some sequence similarities among them.
650	1	4	_aSACCHAROMYCES CEREVISIAE
650	1	4	_aYEAST
650	1	4	_aCELL WALL PROTEIN
650	1	4	_aGPI-ANCHORED PROTEIN
700	1	2	_aHamada, K.
700	1	2	_aFukuchi, S.
700	1	2	_aArisawa, M.
700	1	2	_aBaba, M.
700	1	2	_aKitada, K.
856	4	0	_uhttps://drive.google.com/file/d/1y2RnaIWWiOAzhndc2QbfqM9nwbN_kP6h/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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