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| 005 | 20250625124657.0 | ||
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| 090 | _aB-7244 | ||
| 245 | 1 | 0 | _aDiversity of the exoproteome of Fusarium graminearum grown on plant cell wall |
| 490 | 0 | _vCurrent Genetics, p.1-14, 2005 | |
| 520 | 3 | _aThe exoproteome of the fungus Fusarium graminearum grown on glucose and on hop (Humulus lupulus, L.)cell wall has been investigated. The culture medium was found to contain a higher quantity of proteins and the proteins are more diverse when the fungus is grown on cell wall. Using both 1D and 2D electrophoresis followed by mass spectrometry analysis and protein identification based on similarity searches, 84 unique proteins were identified in the cell wall-grown fungal exoproteome. Many are putatively implicated in carbohydrate metabolism, mainly in cell wall polysaccharide degradation. The predicted carbohydrate-active enzymes fell into 24 different enzymes classes, and up to eight different proteins within a same class are secreted. This indicates that fungal metabolism becomes oriented towards synthesis and secretion of a whole arsenal of enzymes able to digest almost the complete plant cell wall. Cellobiohydrolase is one of the only four proteins found both after growth on glucose and on plant cell wall and we propose that this enzyme could act as a sensor of the extracellular environment. Extensive knowledge of this very diverse F. graminearum exoproteome is an important step towards the full understanding of Fusarium/plants interactions. | |
| 650 | 1 | 4 | _aFUNGI |
| 650 | 1 | 4 | _aFUSARIUM GRAMINEARUM |
| 650 | 1 | 4 | _aHYDROLASES |
| 650 | 1 | 4 | _aPLANT CELL WALL |
| 650 | 1 | 4 | _aPOLYSACCHARIDE DEGRADATION |
| 700 | 1 | 2 | _aPhalip, V. |
| 700 | 1 | 2 | _aDelalande, F. |
| 700 | 1 | 2 | _aCarapito, C: |
| 700 | 1 | 2 | _aGoubet, F. |
| 700 | 1 | 2 | _aHatsch, D. |
| 700 | 1 | 2 | _aLeize-Wagner, E. |
| 700 | 1 | 2 | _aDupree, P. |
| 700 | 1 | 2 | _aDupree, P. |
| 700 | 1 | 2 | _aJeltsch, J.M. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1vWQPRnf41LKDtG8kLwD8-qhAwLyuJxx0/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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