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245	1	0	_aCharacterisation of CwpA, a putative glycosylphosphatidylinositolanchored cell wall mannoprotein in the Wlamentous fungus Aspergillus niger
490	0		_vFungal Genetics and Biology, 42(10), p.873-885, 2005
520	3		_aGlycosylphosphatidylinositol (GPI)-anchored proteins in fungi are found at the cell surface, either as plasma membrane proteins (GPI-PMPs)or attached by a remnant of the GPI-anchor to the cell wall (GPI-CWPs). GPI-CWPs can be extracted from the cell wall by treatment with hydroXuoric acid (HF), which cleaves the phosphodiester bond that is present in the remnant of the GPIanchor. The Wlamentous fungus Aspergillus niger contains at least seven HF-extractable cell wall mannoproteins. One gene encoding an HF-extractable cell wall mannoprotein, cwpA, was cloned and further characterised. The protein sequence of CwpA indicated the presence of two hydrophobic signal sequences both at the N-terminus and C-terminus of the protein, for entering the ER and the addition of a GPI-anchor, respectively. A CwpA-speciWc antiserum was raised and in combination with fractionation experiments, we show that this protein was abundantly present as an HF-extractable protein in the cell wall of A. niger. ÆÉ 2005 Elsevier Inc. All rights reserved.
650	1	4	_aCELL WALL INTEGRITY
650	1	4	_aGPI-ANCHORING
650	1	4	_aCALCOXUOR WHITE
650	1	4	_aHYPERSENSITIVE
650	1	4	_aHF-EXTRACTIONS
650	1	4	_aBETA-SITE
650	1	4	_aGLUCAN
700	1	2	_aDamveld, R.A.
700	1	2	_aArentshorst, M.
700	1	2	_aVankuyk, P.A.
700	1	2	_aKlis, F.M.
700	1	2	_aVan Den Hondel, C.A.M.J.J.
700	1	2	_aRama, A.F.J.
856	4	0	_uhttps://drive.google.com/file/d/1k5V9oc2XeVMCipISDjokYD5tfKRT_Omb/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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