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090			_aB-7516
245	1	0	_aGene silencing using a heat-inducible RNAi system in Arabidopsis
490	0		_vBioChemical and Biophysical Research Communications, 321(2), p.364-369, 2004
520	3		_aControlling gene expression during plant development is an efficient tool to explore gene function. In this paper, we describe a gene expression system driven by a heat-shock gene promoter (HSP18.2), to trigger the expression of an intron-containing invertedrepeat. RNA interference became a powerful way for gene functional analysis by reverse genetic approaches. However, constitutive gene silencing cannot be used with genes involved in fundamental processes such as embryo viability. Inducible promoters provide an alternative approach for temporal and spatial gene expression control and we described here a new system, complementary to those using chemical gene inducers. To evaluate the efficiency of this system, RNA corresponding to the phytoene desaturase gene of Arabidopsis thaliana was used as a reporter gene in transgenic plants and a comparative study was performed using either the CaMV35S constitutive promoter or the HSP18.2 inducible promoter.
650	1	4	_aARABIDOPSIS
650	1	4	_aCONDITIONAL GENE EXPRESSION
650	1	4	_aHEAT-SHOCK
650	1	4	_aPHYTOENE DESATURASE
650	1	4	_aRNA INTERFERENCE
700	1	2	_aMasclaux, F.
700	1	2	_aCharpenteau, M.
700	1	2	_aTakahashi, T.
700	1	2	_aPont-Lezica, R.
700	1	2	_aGalaud, J.-P.
856	4	0	_uhttps://drive.google.com/file/d/1orf--k1iTTn14_zRcM49g9CQ9x6UWWaY/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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