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090 _aB-7524
245 1 0 _aLaser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis
490 0 _vThe Plant Journal, 42(1), p.111-123, 2005
520 3 _aIt is during embryogenesis that the body plan of the developing plant is established. Analysis of gene expression during embryogenesis has been limited due to the technical difficulty of accessing the developing embryo. Here we demonstrate that laser capture microdissection can be applied to the analysis of embryogenesis. We show how this technique can be used in concert with DNA microarray for the large-scale analysis of gene expression in apical and basal domains of the globular-stage and heart-stage embryo, respectively, when critical events of polarity, symmetry and biochemical differentiation are established. This high resolution spatial analysis shows that up to approximately 65
650 1 4 _aARABIDOPSIS THALIANA
650 1 4 _aLASER CAPTURE MICRODISSECTION
650 1 4 _aEMBRYOGENESIS
650 1 4 _aDNA MICROARRAY
650 1 4 _aTRANSCRIPTOME
650 1 4 _aGENE EXPRESSION.
700 1 2 _aCasson, S.
700 1 2 _aSpencer, M.
700 1 2 _aWalker, K.
700 1 2 _aLindsey, K.
856 4 0 _uhttps://drive.google.com/file/d/1foW7yHsWPHkoPJsuhb_0nbTN4OW4rZwV/view?usp=drivesdk
_zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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