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090			_aB-7582
245	1	0	_aIsolation of Nucleic Acids from Plants by Differential Solvent Precipitation
490	0		_vAnalytical BioChemistry, 195, p.45-50, 1991
520	3		_aThe purification of nucleic acids from plant tissue is often made difficult by the presence of contaminating carbohydrate polymers and polyphenols. A procedure for the simultaneous isolation of DNA and translatable RNA from plants is described. The method removes most of the polysaccharides and polyphenols extracted with nucleic acids in a single step by taking advantage of differences in solubility of these compounds in the solvent 2-butoxyethanol. Stepwise addition of 2-butoxyethanol to phenol extracts of specific ionic strength precipitates nucleic acids largely free of contaminants. Subsequent separation of RNA from DNA by precipitation with LiCl was optimised to give a high recovery of translationally active RNA. Successful isolation of nucleic acids from strawberry (Fmgaria X ananassa)receptacle, a particularly recalcitrant tissue, and from a range of tissues of other plant species demonstrates the eneral applicability of the method.
700	1	2	_aManning, K.
856	4	0	_uhttps://drive.google.com/file/d/1lUpOM2p694oTOuE_bPe_nHt1NeMh-xly/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx
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