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| 003 | MX-MdCICY | ||
| 005 | 20250625124706.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-7710 | ||
| 245 | 1 | 0 | _aThe a-tubulin gene AmTuba1: a marker for rapid mycelial growth in the ectomycorrhizal basidiomycete Amanita muscaria |
| 490 | 0 | _vCurrent Genetics, 49(5), p.294-301, 2006 | |
| 520 | 3 | _aThe apical extension of hyphae is of central importance for extensive spread of fungal mycelium in forest soils and for effective ectomycorrhiza development. Since the tubulin cytoskeleton is known to be important for fungal tip growth, we have investigated the expression of an a-tubulin gene from the ectomycorrhizal basidiomycete Amanita muscaria (AmTuba1). The phylogenetic analysis of protein sequences revealed the existence of two subgroups of a-tubulins in homobasidiomycetes, clearly distinguishable by defined amino acids. AmTuba1 belongs to subgroup1. The AmTuba1 transcript level is related to mycelial growth rate. Growth induction of carbohydrate tarved (nongrowing)hyphae resulted in an enhanced AmTuba1 expression as soon as hyphal growth started, reaching a maximum at highest mycelial growth rate. Bacteriuminduced hyphal elongation also leads to increased AmTuba1 transcript levels. In mature A. muscaria/ P. abies ectomycorrhizas, where fungal hyphae are highly branched, and slowly growing, AmTuba1 expression were even lower than in carbohydratestarved mycelium, indicating a further down-regulation of gene expression in symbiosis. In conclusion, our analyses show that the AmTuba1 gene can be used as a marker for active apical extension in fly agaric, and that a-tubulin proteins are romising tools for the classification of fungi. | |
| 650 | 1 | 4 | _aCYTOSKELETON |
| 650 | 1 | 4 | _aECTOMYCORRHIZA |
| 650 | 1 | 4 | _aFILAMENTOUS FUNGI |
| 650 | 1 | 4 | _aTIP GROWTH |
| 650 | 1 | 4 | _aBASIDIOMYCETE |
| 700 | 1 | 2 | _aTarkka, M.T. |
| 700 | 1 | 2 | _aSchrey, S. |
| 700 | 1 | 2 | _aNehls, U. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1v3Icvqm8JEjnnenpL4r0xrK2r3iWIyM4/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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